Symmetrical PAM-PAM prespacer bound Cas4/Cas1/Cas2 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7mi4
Q-score: 0.492
Hu C, Almendros C, Nam KH, Costa AR, Vink JNA, Haagsma AC, Bagde SR, Brouns SJJ, Ke A
Nature (2021) 598 pp. 515-520 [ DOI: doi:10.1038/s41586-021-03951-z Pubmed: 34588691 ]
- Crispr-associated exonuclease cas4/endonuclease cas1 fusion (62 kDa, Protein from Geobacter sulfurreducens)
- Crispr-associated endoribonuclease cas2 (11 kDa, Protein from Geobacter sulfurreducens)
- Dna (35-mer) (10 kDa, DNA from Geobacter sulfurreducens)
- Manganese (ii) ion (54 Da, Ligand)
- Symmetrical pam-pam prespacer bound cas4/cas1/cas2 complex (300 kDa, Complex from Geobacter sulfurreducens)
- Iron/sulfur cluster (351 Da, Ligand)
Asymmetrical PAM-Non PAM prespacer bound Cas4/Cas1/Cas2 complex
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 7mi5
Q-score: 0.318
Hu C, Almendros C, Nam KH, Costa AR, Vink JNA, Haagsma AC, Bagde SR, Brouns SJJ, Ke A
Nature (2021) 598 pp. 515-520 [ DOI: doi:10.1038/s41586-021-03951-z Pubmed: 34588691 ]
- Crispr-associated exonuclease cas4/endonuclease cas1 fusion (62 kDa, Protein from Geobacter sulfurreducens)
- Crispr-associated endoribonuclease cas2 (11 kDa, Protein from Geobacter sulfurreducens)
- Manganese (ii) ion (54 Da, Ligand)
- Dna (34-mer) (11 kDa, DNA from Geobacter sulfurreducens)
- Dna (26-mer) (11 kDa, DNA from Geobacter sulfurreducens)
- Asymmetrical pam-pam prespacer bound cas4/cas1/cas2 complex (300 kDa, Complex from Geobacter sulfurreducens)
- Iron/sulfur cluster (351 Da, Ligand)
Full integration complex of Cas1/Cas2 from Cas4-containing system
Resolution: 3.89 Å
EM Method: Single-particle
Fitted PDBs: 7mi9
Q-score: 0.201
Hu C, Almendros C, Nam KH, Costa AR, Vink JNA, Haagsma AC, Bagde SR, Brouns SJJ, Ke A
Nature (2021) 598 pp. 515-520 [ DOI: doi:10.1038/s41586-021-03951-z Pubmed: 34588691 ]
- Crispr-associated exonuclease cas4/endonuclease cas1 fusion (62 kDa, Protein from Geobacter sulfurreducens)
- Dna (80-mer) (24 kDa, DNA from Geobacter sulfurreducens)
- Crispr-associated endoribonuclease cas2 (11 kDa, Protein from Geobacter sulfurreducens)
- Dna (5'-d(p*gp*ap*ap*gp*cp*c)-3') (1 kDa, DNA from Geobacter sulfurreducens)
- Local refinement half map (spacer side) (Complex)
- Low resolution map before local refinement (Complex)
- Full integration complex of cas1/cas2 from cas4-containing system (300 kDa, Complex from Geobacter sulfurreducens)
- Dna (5'-d(p*cp*gp*gp*ap*ap*ap*ap*gp*ap*gp*cp*c)-3') (3 kDa, DNA from Geobacter sulfurreducens)
- Local refinement half map (leader side) (Complex)
- Dna (72-mer) (22 kDa, DNA from Geobacter sulfurreducens)
Half integration complex of Cas4/Cas1/Cas2 with Cas4 still on the Non-PAM side
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 7mib
Q-score: 0.121
Hu C, Almendros C, Nam KH, Costa AR, Vink JNA, Haagsma AC, Bagde SR, Brouns SJJ, Ke A
Nature (2021) 598 pp. 515-520 [ DOI: doi:10.1038/s41586-021-03951-z Pubmed: 34588691 ]
- Crispr-associated exonuclease cas4/endonuclease cas1 fusion (62 kDa, Protein from Geobacter sulfurreducens)
- Crispr-associated endoribonuclease cas2 (11 kDa, Protein from Geobacter sulfurreducens)
- Half integration complex of cas4/cas1/cas2 and cas4 still in the pam side (300 kDa, Complex from Geobacter sulfurreducens)
- Dna (31-mer) (9 kDa, DNA from Geobacter sulfurreducens)
- Dna (45-mer) (13 kDa, DNA from Geobacter sulfurreducens )
- Dna (64-mer) (19 kDa, DNA from Geobacter sulfurreducens)
- Dna (5'-d(p*cp*gp*gp*ap*ap*ap*ap*gp*ap*gp*cp*c)-3') (3 kDa, DNA from Geobacter sulfurreducens)
Sub-complex of Cas4-Cas1-Cas2 bound PAM containing DNA
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 7mid
Q-score: 0.316
Hu C, Almendros C, Nam KH, Costa AR, Vink JNA, Haagsma AC, Bagde SR, Brouns SJJ, Ke A
Nature (2021) 598 pp. 515-520 [ DOI: doi:10.1038/s41586-021-03951-z Pubmed: 34588691 ]
- Crispr-associated exonuclease cas4/endonuclease cas1 fusion (62 kDa, Protein from Geobacter sulfurreducens)
- Dna (33-mer) (11 kDa, DNA from Geobacter sulfurreducens)
- Crispr-associated endoribonuclease cas2 (11 kDa, Protein from Geobacter sulfurreducens)
- Manganese (ii) ion (54 Da, Ligand)
- Sub-complex of cas4-cas1-cas2 bound pam-containing dna (300 kDa, Complex from Geobacter sulfurreducens)
- Dna (5'-d(p*cp*ap*cp*cp*ap*tp*cp*gp*tp*gp*ap*gp*gp*cp*cp*tp*cp*ap*gp*cp*tp*ap*cp*g)-3') (11 kDa, DNA from Geobacter sulfurreducens)
- Iron/sulfur cluster (351 Da, Ligand)
Half integration complex of Cas1-Cas2 in Cas4 containing system
Hu C, Almendros C, Nam KH, Costa AR, Vink JNA, Haagsma AC, Bagde SR, Brouns SJJ, Ke A
Nature (2021) 598 pp. 515-520 [ DOI: doi:10.1038/s41586-021-03951-z Pubmed: 34588691 ]